Digital Revolution

Single Cell Sequencing Is Coming

Begin

Digital Revolution

Single Cell Sequencing Is Coming

Begin

“If you know your enemy and yourself, you will surely win a war.”

Sun Tzu

About Us

Digenomix is formally spun off from Fluidigm Corporation and now exclusively owns the BIG (Barcode-In-Genome) technology, a novel single cell library preparation platform for NGS (Next-Gen Sequencing). The BIG libraries can be used for copy number determination and mutation detection from a whole genome or from targeted regions with unparalleled precision.

“Put it in a simple way: Others do cancer genome sequencing on bulk level, BIG does it with single cell resolution; Others do in an analog way, BIG does it digitally.“ said Dr. Larry Xi, the founder and CEO of Digenomix.

About BIG

BIG (Barcode-In-Genome) technology, a novel single cell library preparation platform. To help fully understand this novel approach, please click our explanatory VIDEO for an intellectually stimulating tour.

Our Technology

Challenge

A central challenge in sequencing single-cell genomes is the accurate determination of point mutations, phasing of these mutations, and identifying copy number variations with few assumptions.

Ideally, this is accomplished under as low sequencing coverage as possible.

Our recently published review article gives a comprehensive overview on current landscape and challenges facing the single-cell genome sequencing. Click here for this article.

Solution

We achieve these goals with BIG (Barcode-In-Genome), a novel library construction and library amplification methodology.

Starting with single cell, genomic DNA is first fragmented with saturated transposition to make a primary library that uniformly covers the whole genome by short fragments. The library is then amplified by a carefully optimized PCR protocol in a uniform and synchronized fashion for next-generation sequencing. Each step of the protocol can be quantitatively characterized. Our shallow sequencing data show that the library is tightly distributed and is useful for the determination of copy number variations. For more in-depth understanding of this technology, please check out the publication here.